[FLK-1+ cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells].
Zhonghua Xin Xue Guan Bing Za Zhi
; 38(12): 1108-12, 2010 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-21215148
ABSTRACT
OBJECTIVE:
To characterize the differential and proliferative activities of FLK-1(+) cells derived from mouse fetal liver.METHOD:
The FLK-1(+) fraction were enriched from the fetal liver using immunomagnetic method and their differential and proliferative activities were examined in culture medium and in vivo via transplantation of FLK-1(+) cells into the inferior pole of the spleen of nine-week-old male C57 BL/6 mice after two-thirds hepatectomy.RESULTS:
In response to growth factors, FLK-1(+) cells expressed typical lineage-specific markers for vascular endothelial cells, smooth muscle cells. Intrahepatic implantation of FLK-1(+) cells resulted in the formation of blood vessels in the fibrous capsule of partially hepatectomized liver.CONCLUSION:
These results indicate that FLK-1(+) cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells and they may serve as potential stem cells for clinical cell-based therapy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Diferenciação Celular
/
Miócitos de Músculo Liso
/
Receptor 2 de Fatores de Crescimento do Endotélio Vascular
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article