[Effects of TGF-ß1 on gene expression of connective tissue growth factor in lung fibroblasts].
Zhongguo Dang Dai Er Ke Za Zhi
; 13(1): 36-9, 2011 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-21251385
ABSTRACT
OBJECTIVE:
To study the effects of transforming growth factor-ß1 (TGF-ß1) on the gene expression of connective tissue growth factor (CTGF) in cultured lung fibroblasts of embryonic rats in vitro.METHODS:
Wistar rats of embryonic 19 days were used for primary culture of lung fibroblasts (LFs). The cells in the experimental group were treated by different concentrations (1, 5 or 10 ng/mL) and different durations (12, 24 or 48 hrs) of TGF-ß1 to stimulate the LFs. The cells in the control group were cultured in serum-free medium. RT-PCR method was applied to detect CTGF mRNA expression in LFs.RESULTS:
Compared with the control group, the levels of CTGF mRNA in LFs in the experimental group increased significantly (P<0.05). CTGF mRNA expression gradually increased with increasing concentration and duration of TGF-ß1 treatment (P<0.05).CONCLUSIONS:
TGF-ß1 can stimulate CTGF gene expression in LFs and increase CTGF gene expression in a dose-and time-dependent manner.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator de Crescimento Transformador beta1
/
Fator de Crescimento do Tecido Conjuntivo
/
Fibroblastos
/
Pulmão
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article