Human adipose derived stroma/stem cells grow in serum-free medium as floating spheres.
Exp Cell Res
; 317(6): 770-80, 2011 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-21255567
ABSTRACT
With the goal of obtaining clinically safe human adipose-derived stroma/stem cells (ASC) and eliminating the use of serum, we have developed a new culture system that allows the expansion of ASC as spheres in a defined medium. These spheres can be passaged several times. They are not only aggregated cells but rather originate from single cells as clonal spheres can be obtained after seeding at very low density and reform clonal spheres after dissociation. These spheres can also revert to monolayer growth when plated in medium containing human plasma and even generate fibroblast-like colonies (CFU-f). Under several differentiation-specific media, spheres-derived ASC maintain their capacity to differentiate into osteoblasts, endothelial cells and adipocytes. These results indicate that human ASC can be maintained in a serum-free 3D culture system, which is of great interest for the expansion in bioreactors of autologous ASC and their use in clinical trials.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo
/
Adipócitos
/
Técnicas de Cultura de Células
/
Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article