Channel catfish CD8α and CD8ß co-receptors: characterization, expression and polymorphism.
Fish Shellfish Immunol
; 30(3): 894-901, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-21272650
ABSTRACT
In this study we report the identification and characterization of channel catfish, Ictalurus punctatus CD8α and CD8ß genes. Both genes encode predicted proteins containing a leader, a immunoglobulin superfamily V domain, a stalk/hinge region, a transmembrane region and a positively charged cytoplasmic tail (CYT) containing the conserved teleost C-X-H motif. Catfish CD8α and CD8ß are encoded as single copy genes and as in other vertebrates exhibit a conserved head to tail synteny; the CD8ß gene is found 14.1kb upstream of the CD8α gene. Both CD8α and CD8ß transcripts showed a low degree of polymorphism. Finally, as determined by q-PCR both CD8α and CD8ß are expressed in various catfish lymphoid tissues with the highest expression observed in thymus from 2 month old catfish-fry. In the future these results will provide the basis for evaluating the role of CD8(+) CTL and other CD8-bearing cells in response to immunization or infection in the catfish.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
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Ictaluridae
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Regulação da Expressão Gênica
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Antígenos CD8
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article