Large scale expression and purification of recombinant RNA in Escherichia coli.
Methods
; 54(2): 267-73, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21320602
ABSTRACT
Stable, folded RNA are involved in many key cellular processes and can be used as tools for biological, pharmacological and/or molecular design studies. However, their widespread use has been somewhat limited by their fragile nature and by the difficulties associated with their production on a large scale, which were limited to in vitro methods. This work reviews the novel techniques recently developed that allow efficient expression of recombinant RNA in vivo in Escherichia coli. Based on the extensive data available on the genetic and metabolic mechanisms of this model organism, conditions for optimal production can be derived. Combined with a large repertoire of RNA motifs which can be assembled by recombinant DNA techniques, this opens the way to the modular design of RNA molecules with novel properties.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
DNA Recombinante
/
RNA
/
Escherichia coli
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article