[Chemical constituents of caulis Marsdeniae tenocissimae].

ABSTRACT

OBJECTIVE: To study the chemical constitutes of caulis Marsdeniae tenocissimae. METHOD: 70% ethanol extracts of caulis M. tenocissimae were isolated and purified by silica gel column chromatography, Sephadex LH-20 chromatography and semi-preparative reversed phase liquid chromatography (RPLC). The compounds were identified on the basis of spectral analysis, including NMR, MS and IR. RESULT: Seven compounds were obtained and identified and their structures were identified as beta-sitosterol (1), condutirol(2), dihydroconduritol(3), betulinic acid(4), lupeol(5), daucosterol(6), 11alpha-O-(2-methylbutyryl)-12beta-O-acetyltenacigeninB(7). CONCLUSION: Compound 4, 5 were isolated from the genus for the first time.