Expression of a human cocaine-sensitive dopamine transporter in Xenopus laevis oocytes.
J Neurochem
; 54(2): 706-8, 1990 Feb.
Article
em En
| MEDLINE
| ID: mdl-2137162
ABSTRACT
The human dopamine transporter was expressed in Xenopus laevis oocytes following injection of mRNA isolated from human brain substantia nigra. The specific accumulation of [3H]dopamine into these oocytes was time and Na+ dependent. Furthermore, [3H]dopamine accumulation was prevented by coincubation of oocytes with dopamine (100 microM) or with the dopamine uptake inhibitors GBR 12909 (1 microM) or cocaine (3 microM). In contrast, oocyte injection of mRNA isolated from human globus pallidus, an area devoid of dopamine neuron perikarya, did not elicit expression of the dopamine transporter. Oocyte expression of the human dopamine transporter can be used for the further characterization and cloning of this low-abundance membrane protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Proteínas de Membrana Transportadoras
/
Xenopus laevis
/
Glicoproteínas de Membrana
/
Proteínas de Transporte
/
Cocaína
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article