Mechanical damage to Gram-negative bacteria by surface plating with the Drigalski-spatula technique.
Int J Food Microbiol
; 146(1): 105-7, 2011 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-21371768
ABSTRACT
Colony counting by spreading bacterial suspensions on plating media by various techniques is of general concern. Comparative studies between hand plating (Drigalski-spatula technique) for different time intervals and spiral plating resulted in significant differences in colony counts. Lower counts of Gram-negative bacteria were obtained by using hand plating for more than 10s, compared with short time hand plating (5s) or spiral plating. Colony counting of Gram-positive bacteria showed no differences between both techniques. Further characterisation of Escherichia coli cells spread with the Drigalski-spatula technique by electron microscopy revealed a large number of damaged cells compared to control samples. The data clearly shows that the mechanical forces during hand plating are sufficient to damage E. coli cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Bacteriológicas
/
Bactérias Gram-Negativas
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article