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Growth of herpes simplex virus and purification of viral DNA.
Latchman, D S; Kemp, L M.
Afiliação
  • Latchman DS; Medical Molecular Biology Unit, University College and Middlesex School of Medicine, London, UK.
Methods Mol Biol ; 8: 191-200, 1992.
Article em En | MEDLINE | ID: mdl-21390712
In order to use herpes simplex virus (HSV) as a vector for the transmission and expression of foreign genes, it is obviously necessary to be able to prepare stocks of the virus and to propagate both HSV and the recombinant viruses derived from it. Similarly, the introduction of foreign genes into the virus will require the purification of viral DNA for use as a cloning vector, and preparation and analysis of viral DNA will also be necessary in order to characterize the recombinant viruses produced during the cloning procedures. This chapter will therefore discuss in turn the procedures used in our laboratory for the growth of HSV, and for the preparation of viral DNA.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 1992 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 1992 Tipo de documento: Article