[Investigation on long-term survival of transplanted bone marrow mesenchymal stem cells in infarcted myocardium of rats].

ABSTRACT

OBJECTIVE: To investigate the quantification and distribution of survival transplanted bone marrow mesenchymal stem cells (BMSCs) in infarcted myocardium of rats. METHODS: BMSCs of male SD rats were isolated, purified and proliferated. Female SD rats were subjected to occlusion of left anterior descending artery, 1.2 × 10(6) BMSCs were injected into the centre zone of the infarct region 1 h after left anterior descending artery occlusion. Myocardial tissue was harvested randomly at 1, 3 and 6 (n = 18) weeks after transplantation. Transplanted cells were labeled with Hoechst33342 (n = 5) and Brdu (n = 5). Sry sequence of Y chromatosome in male rat was analyzed by real-time PCR in the tissue (n = 8). RESULTS: FACS analysis showed that BMSCs expressed homogenous with CD44+/CD34-. Hoechst33342 or Brdu labeled positive cells were found in localized accumulation and some of them migrated to the margin of the infarct region, and vessel-like structure could be observed in the host hearts. The real-time PCR demonstrated that the survival rates of engrafted BMSCs were 7.88%, 7.82% and 8.73% respectively at 1, 3 and 6 weeks after transplantation. CONCLUSION: BMSCs could survive in the host infarcted myocardium for more than one month and the survival rate was around 8% from the first week to the sixth week after transplantation.