Detection of chromosome abnormalities using cytoplasmic immunoglobulin staining and FISH in myeloma.
Methods Mol Biol
; 730: 159-71, 2011.
Article
em En
| MEDLINE
| ID: mdl-21431641
ABSTRACT
The low proliferation rate of myeloma cells in vitro can result in a normal cytogenetic karyotype with the abnormal cell population not being detected. Because plasma cell myeloma is a patchy disease, conventional FISH is also hampered by normal cell contamination. Identification of plasma cells by cytoplasmic immunoglobulin staining in combination with FISH (cIg FISH) can ensure that only the cells of interest are analyzed, and thus the results obtained are a more accurate reflection of the plasma cell population karyotype. Current literature suggests that probes for t(4;14), t(14;16), and del(17)(p13) should be used in routine diagnostic testing; however, this technique can be used for any probes of interest. In this chapter, we present the techniques and methods used in our laboratory for the detection of abnormalities in plasma cells by cIg staining in conjunction with FISH.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Imunoglobulinas
/
Aberrações Cromossômicas
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Hibridização in Situ Fluorescente
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Citoplasma
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Mieloma Múltiplo
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article