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A comparison of recombinant receptor-reporter gene bioassays and a total estrogen enzyme linked immunosorbent assay for the rapid screening of estrogenic activity in natural and waste waters.
Allinson, Mayumi; Shiraishi, Fujio; Allinson, Graeme.
Afiliação
  • Allinson M; CAPIM, Bio 21 Institute, University of Melbourne, Parkville, VIC 3052, Australia.
Bull Environ Contam Toxicol ; 86(5): 461-4, 2011 May.
Article em En | MEDLINE | ID: mdl-21442212
ABSTRACT
In this study, we compared the performance as screening tools of two yeast-based recombinant receptor-reporter gene bioassays with a commercial ELISA kit for measurement of total estrogens. For WWTP effluents there was a very good correlation between the measured total estrogen concentrations (ELISA) and estrogenic activity by the hERα bioassay (r(2)=0.93), but not for the medERα bioassay (r(2)=0.50). For freshwater samples, the correlations between bioassay response and ELISA ES measurements were very good (r(2)>0.95). There was no correlation between bioassay response and ELISA ES measurements for estuarine samples.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Monitoramento Ambiental / Estrogênios / Disruptores Endócrinos Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Ano de publicação: 2011 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Monitoramento Ambiental / Estrogênios / Disruptores Endócrinos Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Ano de publicação: 2011 Tipo de documento: Article