Employing site-specific recombination for conditional genetic analysis in Sinorhizobium meliloti.
Appl Environ Microbiol
; 77(12): 3916-22, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21515717
ABSTRACT
The ability to remove a genetic function from an organism with good temporal resolution is crucial for characterizing essential genes or genes that act in complex developmental programs. The rhizobium-legume symbiosis involves an elaborate two-organism interaction requiring multiple levels of signal exchange. As an important step toward probing rhizobium genetic functions with temporal resolution, we present the development of a conditional gene deletion system in Sinorhizobium meliloti that employs Cre/loxP site-specific recombination. This system enables chemically inducible and irreversible gene deletion or gene upregulation. Recombinase-mediated excision events can be positively or negatively selected or monitored by a colorimetric assay. The system may be adaptable to various bacterial species, in which recombinase activity may be placed under the control of diverse user-defined promoters. This system also shows promise for uses in promoter trapping and biosensing applications.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinorhizobium meliloti
/
Técnicas de Inativação de Genes
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Genética Microbiana
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article