D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine.
Nat Chem Biol
; 7(8): 528-30, 2011 Apr 27.
Article
em En
| MEDLINE
| ID: mdl-21525873
ABSTRACT
D-ornithine has previously been suggested to enhance the expression of pyrrolysine-containing proteins. We unexpectedly discovered that uptake of D-ornithine results in the insertion of a new amino acid, pyrroline-carboxy-lysine (Pcl) instead of the anticipated pyrrolysine (Pyl). Our feeding and biochemical studies point to specific roles of the poorly understood Pyl biosynthetic enzymes PylC and PylD in converting L-lysine and D-ornithine to Pcl and confirm intermediates in the biosynthesis of Pyl.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ornitina
/
Lisina
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article