New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase.
Biochem Biophys Res Commun
; 408(4): 701-6, 2011 May 20.
Article
em En
| MEDLINE
| ID: mdl-21539815
ABSTRACT
The consecutive genes BF0771-BF0774 in the genome of Bacteroides fragilis NCTC 9343 were found to constitute an operon. The functional analysis of BF0772 showed that the gene encoded a novel enzyme, mannosylglucose phosphorylase that catalyzes the reaction, 4-O-ß-d-mannopyranosyl-d-glucose+Piâmannose-1-phosphate+glucose. Here we propose a new mannan catabolic pathway in the anaerobe, which involves 1,4-ß-mannanase (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772), finally progressing to glycolysis. This pathway is distributed in microbes such as Bacteroides, Parabacteroides, Flavobacterium, and Cellvibrio.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Bacteroides fragilis
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Dissacarídeos
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Genes Bacterianos
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Glucose
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Fosforilases
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Mananas
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article