[Analysis of T-DNA integration pattern in Botrytis cinerea genome].
Wei Sheng Wu Xue Bao
; 51(2): 203-7, 2011 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-21574381
ABSTRACT
OBJECTIVE:
To analyze the T-DNA integration pattern in the genome of grey mold Botrytis cinerea.METHODS:
T-DNA (Transfer DNA) inserted mutant library of Botrytis cinerea was created by Agrobactirium tumfacience mediated transformation. By using TAIL-PCR (Thermal asymmetric interlaced polymerase chain reaction), we amplified and cloned the chromosomal regions flanking T-DNA insertions. The obtained T-DNA flanking sequences were subjected to alignment with standard T-DNA border sequence for identification and analysis of integration.RESULTS:
Up to 69% T-DNA inserted at noncoding regions and 30% inserted at exons. Recombination including deletion or addition of bases in T-DNA region was observed. The right borders of the T-DNA were frequently truncated, and by contrast the left borders were less prone to degradation and appeared to have been inserted in a relatively integrated manner. Extra sequence additions also occurred in T-DNA integration sites.CONCLUSION:
Analysis of T-DNA integration pattern in B. cinerea genome will stimulate the functional genomics study of this fungus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
Genoma Bacteriano
/
Botrytis
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article