p53 regulates Ki-67 promoter activity through p53- and Sp1-dependent manner in HeLa cells.
Tumour Biol
; 32(5): 905-12, 2011 Oct.
Article
em En
| MEDLINE
| ID: mdl-21611785
ABSTRACT
The expression of the human Ki-67 protein, which is strictly associated with cell proliferation, is regulated by a variety of cellular mediators. In this study, we studied the effects of p53 on Ki-67 promoter in HeLa cells using luciferase reporter assay. The results showed that (1) p53 inhibited Ki-67 promoter activity in a dose-dependent manner, (2) the p53-binding motifs mediated part of the transcriptional repression of Ki-67 promoter through a sequence-specific interaction with p53, (3) p53 was able to repress the Sp1-stimulated Ki-67 promoter activity, and (4) the Sp1-binding sites were responsible for the p53-mediated transcriptional repression of Ki-67 promoter. In conclusion, p53 inhibited Ki-67 promoter activity via p53- and Sp1-dependent pathways, and the interaction between p53 and Sp1 might be involved in the transcriptional regulatory mechanisms.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
/
Proteína Supressora de Tumor p53
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Fator de Transcrição Sp1
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Antígeno Ki-67
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article