Directed evolution of an error-prone T7 DNA polymerase that attenuates viral replication.
Chembiochem
; 12(10): 1551-8, 2011 Jul 04.
Article
em En
| MEDLINE
| ID: mdl-21626635
ABSTRACT
Experimental evidence exists that RNA viruses replicate with extremely high mutation rates that result in significant genetic diversity. The diverse nature of viral populations allows rapid adaptation to dynamic environments, and evolution of resistances to vaccines as well as antiviral substances. For DNA viruses that replicate at much greater fidelities, as yet, neither diverse structures in the population nor their responses to increased mutation rates have been sufficiently described. By using the example of DNA bacteriophage T7, we describe the identification of virus-specific DNA polymerase variants with decreased replication fidelities, and their impact on the efficiency of the viral infection cycle.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
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Bacteriófago T7
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Evolução Molecular Direcionada
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DNA Polimerase Dirigida por DNA
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article