Subunit structure and localization of dihydropyridine-sensitive calcium channels in mammalian brain, spinal cord, and retina.
Neuron
; 4(6): 819-32, 1990 Jun.
Article
em En
| MEDLINE
| ID: mdl-2163262
ABSTRACT
Monoclonal antibodies that recognize the alpha 2 delta subunits of calcium channels from skeletal muscle immunoprecipitate a complex of alpha 1, alpha 2 delta, beta, and gamma subunits. They also immunoprecipitate 64% of rabbit brain dihydropyridine-sensitive calcium channels. Iodination of partially purified brain calcium channels followed by immunoprecipitation reveals alpha 1-, alpha 2 delta-, and beta-like subunits that have apparent molecular masses of 175, 142, and 57 kd, respectively. A polypeptide of 100 kd is also specifically immunoprecipitated. Immunocytochemical studies identify dihydropyridine-sensitive calcium channels in neuronal somata and proximal dendrites in rat brain, spinal cord, and retina. Staining of many neuronal somata is uneven, revealing relatively high densities of dihydropyridine-sensitive calcium channels at the base of major dendrites. L-type calcium channels in this location may serve to mediate long-lasting increases in intracellular calcium in the cell body in response to excitatory inputs to the dendrites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Medula Espinal
/
Encéfalo
/
Di-Hidropiridinas
/
Canais de Cálcio
/
Proteínas de Membrana
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article