An insertion vector for the analysis of gene expression during herpes simplex virus infection.
Gene
; 89(2): 271-4, 1990 May 14.
Article
em En
| MEDLINE
| ID: mdl-2165021
ABSTRACT
A herpes simplex virus type 1 (HSV-1) insertion vector, pGal8, was designed for analysis of herpesvirus promoters during virus infection. This vector contains a multiple cloning site (MCS) positioned at the 5' end of the lacZ gene for the insertion of promoter sequences. The MCS and lacZ are flanked by sequences from the HSV-1 thymidine kinase encoding gene (tk) to direct homologous recombination into the tk locus of the viral genome. The utility of this vector is demonstrated by construction and comparison of recombinant viruses that express lacZ from the promoters of the genes encoding glycoprotein C, glycoprotein H and glycoprotein E.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Elementos de DNA Transponíveis
/
Expressão Gênica
/
Transformação Celular Viral
/
Regiões Promotoras Genéticas
/
Simplexvirus
/
Vetores Genéticos
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article