Poly(I:C)-induced tumour cell death leads to DC maturation and Th1 activation.
Cancer Immunol Immunother
; 60(11): 1609-24, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-21691724
ABSTRACT
Dendritic cells (DCs) have the ability to generate peptide epitopes for MHC class I molecules derived from apoptotic tumour cells for direct recognition by cytotoxic T cells. This function has lead to DCs being used in vaccine strategies. In this study, we investigate the effect of inducing apoptosis in tumour cell lines using IFN-γ and poly(IC), the subsequent maturation of the endocytosing DC and its ability to direct the resulting T cell response. We show that uptake of poly(IC)-induced apoptotic tumour cells leads to DC maturation and activation with a Th1 cell polarising capacity. In contrast, these effects are not seen by DCs loaded with γ-irradiated apoptotic tumour cells. We propose that the manner in which tumour cells are induced to die can have a profound effect on the endocytosing DC and the resulting T cell response.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Linfócitos T
/
Poli I-C
/
Células Th1
/
Melanoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article