Co-localization of neural cell adhesion molecule and fibroblast growth factor receptor 2 in early embryo development.
Int J Dev Biol
; 55(3): 313-9, 2011.
Article
em En
| MEDLINE
| ID: mdl-21710437
ABSTRACT
During development there is a multitude of signaling events governing the assembly of the developing organism. Receptors for signaling molecules such as fibroblast growth factor receptor 2 (FGFR2) enable the embryo to communicate with the surrounding environment and activate downstream pathways. The neural cell adhesion molecule (NCAM) was first characterized as a cell adhesion molecule highly expressed in the nervous system, but recent studies have shown that it is also a signaling receptor. Using a novel single oocyte adaptation of the proximity ligation assay, we here show a close association between NCAM and FGFR2 in mouse oocytes and 2-cell embryos. Real-time PCR analyses revealed the presence of messenger RNA encoding key proteins in downstream signaling pathways in oocytes and early mouse embryos. In summary these findings show a co-localization of NCAM and FGFR2 in early vertebrate development with intracellular signaling pathways present to enable a cellular response.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígeno CD56
/
Embrião de Mamíferos
/
Receptor Tipo 2 de Fator de Crescimento de Fibroblastos
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article