High-sensitive protein analysis by FESI-CE-MALDI-MS.
Electrophoresis
; 32(14): 1795-803, 2011 Jul.
Article
em En
| MEDLINE
| ID: mdl-21710548
ABSTRACT
Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iontophoretic fraction collection interface for the coupling of CE and MALDI-MS, the potential of the combination of both techniques for the analysis of intact proteins is assessed. To further provide a bioanalytical platform with high-sensitivity capabilities, field-enhanced sample injection is integrated as on online preconcentration strategy upstream from the electrokinetic separation. Under optimized conditions, more than 3200- and 4800-fold improvement, respectively in terms of peak height and peak area, as well as LODs ranging from 5 to 10 nM, has been achieved.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Eletroforese Capilar
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article