Differential expression profile of membrane proteins in zebrafish (Danio rerio) brain exposed to methyl parathion.
Proteomics
; 11(18): 3743-56, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21761560
ABSTRACT
Methyl parathion (MP) is a widely used organophosphorus pesticide, which has been related to a broad spectrum of toxic effects on environmental organisms. The present study investigated the changes in the protein profile of enriched membrane fraction from zebrafish (Danio rerio) brain exposed to three concentrations (0.5, 1 and 2 mg/L) of MP. 2-DE revealed that the abundance of 21 protein spots was significantly changed by MP stress. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database search, 16 protein spots were identified as membrane proteins, among which 8 were down-regulated, while 8 were up-regulated. These proteins are mainly involved in oxidative stress response, signal transduction, metabolism, protein synthesis and degradation, neuroplasticity and regeneration as well as synaptic transmission. These results may aid our understanding of the mechanism of MP-induced neurotoxicity and provide the possibility of the establishment of candidate biomarkers of MP.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
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Encéfalo
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Proteínas de Peixe-Zebra
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Transcriptoma
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Proteínas de Membrana
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Metil Paration
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article