Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy.
Bioorg Med Chem Lett
; 21(18): 5347-52, 2011 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-21802945
ABSTRACT
Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5]oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Purinas
/
Proteínas de Choque Térmico HSP90
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article