[Effect and mechanism of matrine on apoptosis of Raji cells].
Zhong Yao Cai
; 34(4): 576-9, 2011 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-21809546
ABSTRACT
OBJECTIVE:
To invest the effect and mechanism of matrine on apoptosis of human Burkitt's lymphoma Raji cells.METHODS:
Raji cells were cultured in vitro and treated by different final concentrations (0.4, 0.8, 1.6 mg/mL) of matrine or combined with SB203580 (p38 MAPK inhibitor) before matrine was added, then cocultured for 48 h, cell apoptosis rate was detected by Annexin V-FITC/PI double staining method and the P-p38 MAPK, Fas, FasL protein expresssion of Raji cells were evaluated by Western blot.RESULTS:
After cells were treated by matrine (0.4, 0.8, 1.6 mg/mL), the corresponding total apoptosis rate (15.77 +/- 0.53)%, (27.88 +/- 1.52)%, (48.08 +/- 2.87)%, had statistical significance compared with SB203580 groups (11. 48 +/- 0.64)%, (19.34 +/- 0.91)%, (33.98 +/- 1.26)% (P < 0.05 or P < 0.01), and control group (8.78 +/- 0.66)% (P < 0.05 or P < 0.01). As the concentration of matrine gradually increased,the protein expresssion levels of P-p38MAPK, Fas, FasL increased, and decreased after SB203580 were added, the correlation of P-p38MAPK and Fas, FasL was obvious.CONCLUSION:
Matrine can upregulation of Fas and FasL to promote the apoptosis of Raji cells, it may be related to p38MAPK Activation.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piridinas
/
Quinolizinas
/
Linfoma de Burkitt
/
Apoptose
/
Alcaloides
/
Imidazóis
/
Antineoplásicos Fitogênicos
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article