Low calcium culture condition induces mesenchymal cell-like phenotype in normal human epidermal keratinocytes.
Biochem Biophys Res Commun
; 412(2): 226-31, 2011 Aug 26.
Article
em En
| MEDLINE
| ID: mdl-21810412
ABSTRACT
Epithelial-mesenchymal transition (EMT) is an important cellular phenomenon in organ developments, cancer invasions, and wound healing, and many types of transformed cell lines are used for investigating for molecular mechanisms of EMT. However, there are few reports for EMT in normal human epithelial cells, which are non-transformed or non-immortalized cells, in vitro. Therefore, normal human epidermal keratinocytes (NHEK) serially cultured in low-calcium concentration medium (LCM) were used for investigating relations between differentiation and proliferation and mesenchymal-like phenotype in the present study, since long-term cultivation of NHEK is achieved in LCM. Interestingly, NHEK serially cultured in LCM consisted essentially of cytokeratin-vimentin double positive cells (98%), although the NHEK exhibited differentiation under high-calcium culture condition with 3T3 feeder layer. The vimentin expression was suppressed under high-calcium condition. These results may indicate the importance of mesenchymal-like phenotype for serially cultivation of NHEK in vitro.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
/
Diferenciação Celular
/
Cálcio
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Transição Epitelial-Mesenquimal
/
Células Epidérmicas
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article