Immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571 using modified sodium alginate beads.
Bioprocess Biosyst Eng
; 35(3): 383-8, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-21833621
ABSTRACT
An experimental design was carried out to evaluate the effect of the concentrations of sodium alginate, glutaraldehyde and activated coal on the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. The experimental condition of 20 g/L of sodium alginate, 50 mL/L of glutaraldehyde and 30 g/L of activated coal led to the highest specific activity (2,063.5 U/mg of protein), corresponding to an enhancement of about 26 times compared to the activity of the free enzyme (79.1 U/mg of protein). The effect of pH and temperature on the immobilized enzyme activity was also evaluated, showing optimal activities at pH of 5.5 and 55 °C. The study of storage of immobilized inulinase in different temperatures showed that the extract kept its initial activity after 43 days of storage at 40 and 50 °C and after 138 days of storage either at 4 or 25 °C.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Kluyveromyces
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Proteínas Fúngicas
/
Alginatos
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Enzimas Imobilizadas
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Glicosídeo Hidrolases
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article