Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry.
J Invertebr Pathol
; 108(2): 126-30, 2011 Oct.
Article
em En
| MEDLINE
| ID: mdl-21843526
ABSTRACT
Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Artrópodes
/
Interações Hospedeiro-Patógeno
/
Citometria de Fluxo
/
Hemócitos
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article