Development of a highly sensitive and specific immunoassay for determining chrysoidine, a banned dye, in soybean milk film.
Molecules
; 16(8): 7043-57, 2011 Aug 17.
Article
em En
| MEDLINE
| ID: mdl-21849932
A highly specific and sensitive indirect competitive enzyme-linked immunosorbent assay (icELISAï¼was developed for the first time for the detection of chrysoidine, a dye banned in soybean milk film. Two haptens with different spacer arms were synthesized to produce antibodies. Both homologous and heterologous immunoassay formats were compared to enhance the icELISA sensitivity. The heterologous icELISA exhibited better performance, with an IC(50) (50% inhibitory concentration) of 0.33 ng/mL, a limit of detection (LOD, 10% inhibitory concentration) of 0.04 ng/mL, and a limit of quantitation (LOQ, 20%-80% inhibitory concentration) from 0.09 to 4.9 ng/mL. The developed icELISA was high sensitive and specific, and was applied to determine chrysoidine in fortified soybean milk film samples. The results were in good agreement with that obtained by high-performance liquid chromatography (HPLC) analyses.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
P-Aminoazobenzeno
/
Glycine max
/
Ensaio de Imunoadsorção Enzimática
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Contaminação de Alimentos
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Emulsões
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article