General approach for monitoring peptide-protein interactions based on graphene-peptide complex.
Anal Chem
; 83(19): 7276-82, 2011 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-21859076
ABSTRACT
Peptide-protein interactions have critical roles in biology. Monitoring peptide-protein interactions plays an important role in investigating molecular recognition, screening drugs, and designing biosensors. In this paper, we develop a novel fluorescent approach to monitor peptide-protein interactions based on the assembly of pyrene-labeled peptide and graphene oxide (GO). The pyrene-labeled peptide is strongly adsorbed on the surface of GO via π-π interactions and hydrophobic interactions. As a result, the proximity of the GO to the pyrene moiety effectively quenches the fluorescence of pyrene. In the presence of target protein, the competitive binding of the target protein with GO for peptide results in the restoration of fluorescence signal. This signaling mechanism makes it possible to monitor the peptide-protein interactions in a homogeneous real-time format.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Proteínas
/
Grafite
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article