Zac1 functional interactions mediate AP-1 transcriptional activity.
Biochim Biophys Acta
; 1813(12): 2050-60, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21864583
A zinc-finger protein which regulates apoptosis and cell cycle arrest 1 (Zac1) is a novel seven-zinc-finger protein that can bind a specific GC-rich DNA element and has intrinsic transactivation activity; therefore, its role as a transcription factor has been proposed. Zac1 not only promotes cell cycle arrest and apoptosis but also acts as a transcriptional cofactor for nuclear receptors and p53. In this study, we examined the functional roles of mouse Zac1 (mZac1) in HeLa cells treated with 12-O-tetradecanoylphorbol-13-acetate (PMA), a potent Activator protein 1 (AP-1) activator. At first, we found that mZac1 prolonged and enhanced PMA-induced AP-1 activity in both HeLa and HeLa/p53 shRNA cells. We further identified physical and functional interactions between mZac1 and AP-1 proteins (either c-Jun, c-Fos or both). Finally, we showed that Zac1 might function as a selective coactivator of AP-1, demonstrated by AP-1-dependent transcriptional activation of collagenase, c-Fos and p21(WAF1/Cip1) promoter activities. Identification of AP-1 as a specific target for Zac1-mediated transcriptional events not only establishes a direct link between these two pivotal regulatory proteins but also raises the possibility that Zac1 contributes to certain AP-1-dependent biological effects.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Regulação Neoplásica da Expressão Gênica
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Proteína Supressora de Tumor p53
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Proteínas Proto-Oncogênicas c-fos
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Proteínas de Ciclo Celular
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Proteínas Supressoras de Tumor
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Proteínas Quinases JNK Ativadas por Mitógeno
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Inibidor de Quinase Dependente de Ciclina p21
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article