Improved specificity for detection of Mycobacterium bovis in fresh tissues using IS6110 real-time PCR.
BMC Vet Res
; 7: 50, 2011 Aug 25.
Article
em En
| MEDLINE
| ID: mdl-21867516
ABSTRACT
BACKGROUND:
Culture of M. bovis from diagnostic specimens is the gold standard for bovine tuberculosis diagnostics in the USA. Detection of M. bovis by PCR in tissue homogenates may provide a simple rapid method to complement bacterial culture. A significant impediment to PCR based assays on tissue homogenates is specificity since mycobacteria other than M. bovis may be associated with the tissues.RESULTS:
Previously published IS6110 based PCR diagnostic assays, along with one developed in house, were tested against environmental mycobacteria commonly isolated from diagnostic tissues submitted to the National Veterinary Services Laboratory. A real-time PCR assay was developed (IS6110_T) that had increased specificity over other IS6110 based assays. Of the 13 non-tuberculous mycobacteria tested with IS6110_T only M. wolinskyi was positive. Thirty M. bovis infected tissue homogenates and 18 control tissues were used to evaluate the potential for the assay as a diagnostic test. In this small sample, IS6110_T detected 20/30 samples from M. bovis infected animals and 0/18 control tissues.CONCLUSIONS:
The IS6110_T assay provides a PCR based assay system that is compatible with current diagnostic protocols for the detection of M. bovis in the USA and compliments current testing strategies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculose Bovina
/
Reação em Cadeia da Polimerase em Tempo Real
/
Mycobacterium bovis
Tipo de estudo:
Diagnostic_studies
/
Guideline
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article