Synthesis of papain in Escherichia coli.
Gene
; 88(2): 263-7, 1990 Apr 16.
Article
em En
| MEDLINE
| ID: mdl-2189787
ABSTRACT
We have transferred the cloned papain genetic information into an expression vector (pT7-7) regulated by the T7-promoter and have obtained in vitro expression as well as expression in Escherichia coli. In Western blots the proteins produced are immunologically recognizable as papain. Multiple forms of specific but differing sizes are detected, suggesting either that initiation can occur at more than one of the upstream methionines, or that the enzyme is processed after synthesis.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papaína
/
Escherichia coli
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article