Legionella steelei sp. nov., isolated from human respiratory specimens in California, USA, and South Australia.
Int J Syst Evol Microbiol
; 62(Pt 8): 1766-1771, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-21948093
ABSTRACT
Legionella-like bacteria were isolated from the respiratory tract of two patients in California, USA, and South Australia, but were not thought to cause disease. These bacteria, strains F2632 and IMVS-3376(T), were found to have identical Legionella macrophage infectivity potentiator (mip) gene sequences and were therefore further characterized to determine their genetic and phenotypic relatedness and properties. Both of these Gram-negative-staining bacterial strains grew on buffered charcoal yeast extract medium, were cysteine auxotrophs and made a characteristic diffusible bright yellow fluorescent pigment, with one strain making a late appearing colony-bound blue-white fluorescent pigment. The optimal in vitro growth temperature was 35 °C, with very poor growth at 37 °C in broth or on solid media. There was no growth in human A549 cells at either 35 or 37 °C, but excellent growth in Acanthamoeba castellani at 30 °C and poorer growth at 35 °C. Phylogenetic analysis of these bacteria was performed by sequence analysis of 16S rRNA, mip, ribonuclease P, ribosomal polymerase B and zinc metalloprotease genes. These studies confirmed that the new strains represented a single novel species of the genus Legionella for which the name Legionella steelei sp. nov. is proposed. The type strain is IMVS-3376(T) (â=âIMVS 3113(T)â=âATCC BAA-2169(T)).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Sistema Respiratório
/
Legionella
Limite:
Adult
/
Humans
/
Male
País/Região como assunto:
America do norte
/
Oceania
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article