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[Location of Dctn1 in the mouse testis and sperm and its role in spermiogenesis].
Zheng, Bo; Jiang, Min; Li, Su-Ying; Wu, Yi-Bo; Zhu, Hui; Zhou, Zuo-Min; Sha, Jia-Hao.
Afiliação
  • Zheng B; Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China. mansnoopy@163.com
Zhonghua Nan Ke Xue ; 17(9): 799-804, 2011 Sep.
Article em Zh | MEDLINE | ID: mdl-21961240
ABSTRACT

OBJECTIVE:

To investigate the role of dynactin 1 (Dctn1) in the process of mouse spermiogenesis.

METHODS:

Western blot and indirect immunofluorescence were used to analyze the expression and location of Dctn1 in the mouse testis and spermatozoa. The highest efficiency of small interference RNA (siRNA) was verified by GC2-spd cell line in vitro and in vivo studies, respectively. Dctn1 siRNA mixed with the indicator (0.4% trypan blue) was injected into the seminiferous tubules of 3-week-old ICR mice through rete testis microinjection, and negative control siRNA injected into the control testes. The normal group included 3-week-old ICR mice that did not receive any treatment. Spermatozoa were collected from the cauda epididymis 3 weeks after siRNA injection for morphological analysis.

RESULTS:

Dctn1 was mainly localized in the tail of spermatozoa. After interference, the sperm tail abnormality in the Dctn1 siRNA group was (23.57 +/- 0.55)%, significantly higher than (12.35 +/- 2.29)% in the control (P < 0.01, n = 3), and it was (3.37 +/- 0.69)% in the normal group.

CONCLUSION:

Dctn1 plays an important role in mouse spermiogenesis, and mainly affects the formation of the tail of spermatozoa.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatogênese / Espermatozoides / Testículo / Proteínas Associadas aos Microtúbulos Limite: Animals Idioma: Zh Ano de publicação: 2011 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatogênese / Espermatozoides / Testículo / Proteínas Associadas aos Microtúbulos Limite: Animals Idioma: Zh Ano de publicação: 2011 Tipo de documento: Article