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Gαi2- and Gαi3-specific regulation of voltage-dependent L-type calcium channels in cardiomyocytes.
Dizayee, Sara; Kaestner, Sonja; Kuck, Fabian; Hein, Peter; Klein, Christoph; Piekorz, Roland P; Meszaros, Janos; Matthes, Jan; Bjrnbaumer, Lutz; Nürnberg, Bernd; Herzig, Stefan.
Afiliação
  • Dizayee S; Department of Pharmacology, University of Cologne, Cologne, Germany.
PLoS One ; 6(9): e24979, 2011.
Article em En | MEDLINE | ID: mdl-21966394
ABSTRACT

BACKGROUND:

Two pertussis toxin sensitive G(i) proteins, G(i2) and G(i3), are expressed in cardiomyocytes and upregulated in heart failure. It has been proposed that the highly homologous G(i) isoforms are functionally distinct. To test for isoform-specific functions of G(i) proteins, we examined their role in the regulation of cardiac L-type voltage-dependent calcium channels (L-VDCC).

METHODS:

Ventricular tissues and isolated myocytes were obtained from mice with targeted deletion of either Gα(i2) (Gα(i2) (-/-)) or Gα(i3) (Gα(i3) (-/-)). mRNA levels of Gα(i/o) isoforms and L-VDCC subunits were quantified by real-time PCR. Gα(i) and Ca(v)α(1) protein levels as well as protein kinase B/Akt and extracellular signal-regulated kinases 1/2 (ERK1/2) phosphorylation levels were assessed by immunoblot analysis. L-VDCC function was assessed by whole-cell and single-channel current recordings.

RESULTS:

In cardiac tissue from Gα(i2) (-/-) mice, Gα(i3) mRNA and protein expression was upregulated to 187 ± 21% and 567 ± 59%, respectively. In Gα(i3) (-/-) mouse hearts, Gα(i2) mRNA (127 ± 5%) and protein (131 ± 10%) levels were slightly enhanced. Interestingly, L-VDCC current density in cardiomyocytes from Gα(i2) (-/-) mice was lowered (-7.9 ± 0.6 pA/pF, n = 11, p<0.05) compared to wild-type cells (-10.7 ± 0.5 pA/pF, n = 22), whereas it was increased in myocytes from Gα(i3) (-/-) mice (-14.3 ± 0.8 pA/pF, n = 14, p<0.05). Steady-state inactivation was shifted to negative potentials, and recovery kinetics slowed in the absence of Gα(i2) (but not of Gα(i3)) and following treatment with pertussis toxin in Gα(i3) (-/-). The pore forming Ca(v)α(1) protein level was unchanged in all mouse models analyzed, similar to mRNA levels of Ca(v)α(1) and Ca(v)ß(2) subunits. Interestingly, at the cellular signalling level, phosphorylation assays revealed abolished carbachol-triggered activation of ERK1/2 in mice lacking Gα(i2).

CONCLUSION:

Our data provide novel evidence for an isoform-specific modulation of L-VDCC by Gα(i) proteins. In particular, loss of Gα(i2) is reflected by alterations in channel kinetics and likely involves an impairment of the ERK1/2 signalling pathway.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP / Canais de Cálcio Tipo L / Miócitos Cardíacos / Subunidade alfa Gi2 de Proteína de Ligação ao GTP Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2011 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP / Canais de Cálcio Tipo L / Miócitos Cardíacos / Subunidade alfa Gi2 de Proteína de Ligação ao GTP Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2011 Tipo de documento: Article