Assay-dependent effect of silver nanoparticles to Escherichia coli and Bacillus subtilis.
Appl Microbiol Biotechnol
; 92(5): 1045-52, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21986863
ABSTRACT
We assess the microbial assay-dependent effect of AgNP on gram-negative Escherichia coli and gram-positive Bacillus subtilis. The experiment was conducted via three different assays a growth inhibition assay, a colony forming unit assay, and a liquid-to-plate assay. AgNP were exposed either as liquid suspensions or in an agar state. Bacterial sensitivity to AgNP was found to be dependent on the microbial assay employed. E. coli was more sensitive than B. subtilis in the growth inhibition and CFU assays, but B. subtilis was more vulnerable than E. coli in the liquid-to-plate assay, ostensibly owing to the food stress mechanisms of B. subtilis in exposure medium. The dissolution of silver from AgNP could not explain the observed toxicity of AgNP. We detected clear evidence of AgNP uptake by cells. The results of this study showed that the microbial toxicity of AgNP and the effects of dissolved silver ions were influenced profoundly by the microbial test method employed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prata
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Bacillus subtilis
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Contagem de Colônia Microbiana
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Técnicas Bacteriológicas
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Escherichia coli
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Nanopartículas Metálicas
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article