Palladium(II) and platinum(II) bind strongly to an engineered blue copper protein.
Inorg Chem
; 50(22): 11294-6, 2011 Nov 21.
Article
em En
| MEDLINE
| ID: mdl-22026434
ABSTRACT
Studies of palladium(II) and platinum(II) binding to well-characterized proteins contribute to understanding the influence of these metals in the environment and body. The well-characterized apoprotein of azurin has a soft-metal binding site that may be exposed to solvent by mutation of a coordinating His-117 residue to glycine (H117G). Palladium(II) and platinum(II) form strong 11 adducts with the apo form of H117G azurin. A combination of UV-vis, circular dichroism, and inductively coupled plasma mass spectrometry techniques suggests that the metal binds specifically at His-46 and Cys-112 of the protein.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paládio
/
Platina
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Pseudomonas aeruginosa
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Azurina
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Proteínas de Bactérias
/
Cobre
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article