Live-cell real-time imaging reveals role of mitochondria in cell-to-cell transmission of HIV-1.
Biochem Biophys Res Commun
; 415(2): 384-9, 2011 Nov 18.
Article
em En
| MEDLINE
| ID: mdl-22037455
ABSTRACT
We used live-cell, real-time fluorescence imaging of co-cultures of HIV-1 infected T cells and uninfected target cells to examine the action of mitochondria during cell-to-cell transmission of the virus. We find that mitochondria of HIV infected cells enter uninfected target cells and advance viral spread. We show that human mitochondria serve as viral reservoirs and carriers and that they can move between cells. This was confirmed by our results that purified mitochondria from HIV infected cells are infectious, and that mitochondrial inhibitors block HIV transmission. Viral infection and replication in the target cells were verified by syncytial formation and HIV-1 core protein p24 production. Our results offer new insights into the cellular mechanisms of viral transmission and identify mitochondria as new host targets for viral infection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Infecções por HIV
/
HIV-1
/
Internalização do Vírus
/
Mitocôndrias
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article