Cross-linking with ultraviolet-a and riboflavin reduces corneal permeability.
Invest Ophthalmol Vis Sci
; 52(12): 9275-8, 2011 Nov 29.
Article
em En
| MEDLINE
| ID: mdl-22064989
ABSTRACT
PURPOSE:
To investigate the effect of cross-linking treatment on corneal permeability in a live animal model.METHODS:
Rabbit eyes were selected at random to be left unoperated or to undergo epithelial debridement with or without treatment consisting of cross-linking (CXL) with riboflavin and ultraviolet-A. Nine eyes received a total dose of 3.6 J/cm² and after epithelial healing the corneas were placed in a two-chamber system for quantification of the diffusion of fluorescein compared with controls. Thirty eyes received a total dose of 5.4 J/cm² and, after epithelial healing, in vivo corneal permeability was quantified as the pupillary response over a 30-minute period to a dose of topical pilocarpine compared with controls.RESULTS:
In the ex vivo assay, the mean permeability coefficient in the CXL group (2.42 × 10â»7) was reduced when compared with the unoperated controls (3.73 × 10â»7; P = 0.007) and to the eyes that received epithelial debridement alone (3.74 × 10â»7; P = 0.01). In the in vivo permeability assay, the change in pupillary diameter at 30 minutes after pilocarpine administration was smaller in the CXL group (-1.9 mm), compared with the epithelial debridement group (-2.6 mm; P < 0.001) and with the unoperated controls (-2.7 mm; P = 0.003).CONCLUSIONS:
Corneal cross-linking with ultraviolet-A and riboflavin results in a statistically significant reduction in corneal permeability. These findings suggest that dosing of topical medications may need to be increased in eyes with a history of CXL to achieve expected therapeutic effects, and they may have implications for the long-term health of the cornea.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Riboflavina
/
Raios Ultravioleta
/
Fármacos Fotossensibilizantes
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Córnea
/
Reagentes de Ligações Cruzadas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article