Myofibroblast persistence and collagen type I accumulation in the human stenotic trachea.
Head Neck
; 34(9): 1283-93, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-22076928
ABSTRACT
BACKGROUND:
Postintubation tracheal stenosis (PITS) is associated with an increased use of assisted ventilation in intensive care units. We investigated both collagen type I accumulation and myofibroblast localization in human PITS lesions excised for surgical therapeutic procedures, compared with normal tracheas.METHODS:
We analyzed 2 segments of normal tracheas and 10 segments of PITS that were stained by hematoxylin-eosin and picrosirius red techniques and processed for immunohistochemistry using antibodies against both α-smooth muscle actin (α-sma) for myofibroblast detection, and collagen type I.RESULTS:
We showed a significant increase in collagen deposition in PITS specimens compared with normal tracheas. We found spindle-shaped α-sma-positive cells (myofibroblasts) in the subepithelial layer of all pathologic tracheas, and the persistence of an intense myofibroblast network at PITS sites.CONCLUSIONS:
Tracheal wall thickening in PITS is due to a deranged collagen remodeling that is related to myofibroblast activation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traqueia
/
Estenose Traqueal
/
Colágeno Tipo I
/
Miofibroblastos
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article