[Establishment of cellular immunity of enhanced hepatitis B vaccine].

ABSTRACT

OBJECTIVE: To establish the method to detect the cellular immune response of enhanced hepatitis B vaccine and make verification preliminary. METHODS: Immunized BALB/c mice with enhanced hepatitis B vaccine and detected the IFN-gamma spots forming cells (SFC) of mouse spleen cell by Elispot. Optimized the conditions of the experiment. Cellular immune response between enhanced hepatitis B vaccine and normal hepatitis B vaccine by Elispot were compared. RESULTS: IFN-gamma SFC was higher in 5microg dose than in 2microg dose after immunization with enhanced hepatitis B vaccine and IFN-gamma SFC was declined after immunization 3 weeks ago. IFN-gamma SFC was higher in stimulus by peptide than by protein. Compared to normal hepatitis B vaccine, IFN-gamma SFC was higher in enhanced hepatitis B vaccine. CONCLUSION: Established the detection method to evaluate the cellular immunity of enhanced hepatitis B vaccine and tested the repeatability.