Repetitive DNA and next-generation sequencing: computational challenges and solutions.
Nat Rev Genet
; 13(1): 36-46, 2011 Nov 29.
Article
em En
| MEDLINE
| ID: mdl-22124482
ABSTRACT
Repetitive DNA sequences are abundant in a broad range of species, from bacteria to mammals, and they cover nearly half of the human genome. Repeats have always presented technical challenges for sequence alignment and assembly programs. Next-generation sequencing projects, with their short read lengths and high data volumes, have made these challenges more difficult. From a computational perspective, repeats create ambiguities in alignment and assembly, which, in turn, can produce biases and errors when interpreting results. Simply ignoring repeats is not an option, as this creates problems of its own and may mean that important biological phenomena are missed. We discuss the computational problems surrounding repeats and describe strategies used by current bioinformatics systems to solve them.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Sequências Repetitivas de Ácido Nucleico
/
Alinhamento de Sequência
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Análise de Sequência de RNA
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Análise de Sequência de DNA
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Biologia Computacional
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article