Biological activities of histidine-rich peptides; merging biotechnology and nanomedicine.
Microb Cell Fact
; 10: 101, 2011 Dec 02.
Article
em En
| MEDLINE
| ID: mdl-22136342
ABSTRACT
Histidine-rich peptides are commonly used in recombinant protein production as purification tags, allowing the one-step affinity separation of the His-tagged proteins from the extracellular media or cell extracts. Genetic engineering makes feasible the post-purification His-tag removal by inserting, between the tag and the main protein body, a target site for trans-acting proteases or a self-proteolytic peptide with regulatable activities. However, for technical ease, His tags are often not removed and the fusion proteins eventually used in this form. In this commentary, we revise the powerful biological properties of histidine-rich peptides as endosomolytic agents and as architectonic tags in nanoparticle formation, for which they are exploited in drug delivery and other nanomedical applications. These activities, generally unknown to biotechnologists, can unwillingly modulate the functionality and biotechnological performance of recombinant proteins in which they remain trivially attached.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Biotecnologia
/
Nanomedicina
/
Histidina
Limite:
Humans
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article