Flaviviral helicase: insights into the mechanism of action of a motor protein.
Biochem Biophys Res Commun
; 417(1): 84-7, 2012 Jan 06.
Article
em En
| MEDLINE
| ID: mdl-22138238
ABSTRACT
Motor proteins are involved in crucial cell activities, such as cargo transport or nucleic acid remodeling, by converting the free energy of ATP hydrolysis into motion or mechanical work. Flavivirus helicase is a motor protein involved in dsRNA separation during viral replication, thus essential for virus infection. Since a clear vision of the protein activity, in particular of the relationship between ATP cycling and dynamics, is missing, we carried over a molecular dynamics study on Dengue virus helicase in its ATP bound and unbound states. Our simulations show different opening levels of the ssRNA access site to the helicase core. Specifically, we show that ATP induces a closed state into the ssRNA access site, likely involved in the helicase unwinding activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Helicases
/
Proteínas Motores Moleculares
/
Vírus da Dengue
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article