Production of multi-fiber modifying enzyme from Mamillisphaeria sp. for refining of recycled paper pulp.
Biosci Biotechnol Biochem
; 75(12): 2297-303, 2011.
Article
em En
| MEDLINE
| ID: mdl-22146707
ABSTRACT
Enzymatic modification of pulp is receiving increasing interest for energy reduction at the refining step of the paper-making process. In this study, the production of a multi-fiber modifying enzyme from Mamillisphaeria sp. BCC8893 was optimized in submerged fermentation using a response-surface methodology. Maximal production was obtained in a complex medium comprising wheat bran, soybean, and rice bran supplemented with yeast extract at pH 6.0 and a harvest time of 7 d, resulting in 9.2 IU/mL of carboxymethyl cellulase (CMCase), 14.9 IU/mL of filter paper activity (FPase), and 242.7 IU/mL of xylanase. Treatment of old corrugated container pulp at 0.2-0.3 IU of CMCase/g of pulp led to reductions in refining energy of 8.5-14.8%. The major physical properties were retained, including tensile and compression strength. Proteomic analysis showed that the enzyme was a complex composite of endo-glucanases, cellobiohydrolases, beta-1,4-xylanases, and beta-glucanases belonging to various glycosyl hydrolase families, suggestive of cooperative enzyme action in fiber modification, providing the basis for refining efficiency.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papel
/
Ascomicetos
/
Celulase
/
Endo-1,4-beta-Xilanases
/
Química Verde
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article