Random insertional mutagenesis in fungal genomes to identify virulence factors.
Methods Mol Biol
; 835: 509-17, 2012.
Article
em En
| MEDLINE
| ID: mdl-22183674
ABSTRACT
Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mutagênese Insercional
/
Genoma Fúngico
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Solanum lycopersicum
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Verticillium
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Fatores de Virulência
Tipo de estudo:
Clinical_trials
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article