Hho1p, the linker histone of Saccharomyces cerevisiae, is important for the proper chromatin organization in vivo.
Biochim Biophys Acta
; 1819(5): 366-74, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22200500
ABSTRACT
Despite the existence of certain differences between yeast and higher eukaryotic cells a considerable part of our knowledge on chromatin structure and function has been obtained by experimenting on Saccharomyces cerevisiae. One of the peculiarities of S. cerevisiae cells is the unusual and less abundant linker histone, Hho1p. Sparse is the information about Hho1p involvement in yeast higher-order chromatin organization. In an attempt to search for possible effects of Hho1p on the global organization of chromatin, we have applied Chromatin Comet Assay (ChCA) on HHO1 knock-out yeast cells. The results showed that the mutant cells exhibited highly distorted higher-order chromatin organization. Characteristically, linker histone depleted chromatin generally exhibited longer chromatin loops than the wild-type. According to the Atomic force microscopy data the wild-type chromatin appeared well organized in structures resembling quite a lot the "30-nm" fiber in contrast to HHO1 knock-out yeast.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Cromatina
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Histonas
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Cromossomos
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Proteínas de Saccharomyces cerevisiae
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article