Localization of linear B-cell epitopes on goose parvovirus structural protein.
Vet Immunol Immunopathol
; 145(1-2): 522-6, 2012 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-22209204
ABSTRACT
Goose parvovirus (GPV), a small non-enveloped ssDNA virus, can cause Derzsy's disease, a highly contagious and lethal disease in goslings and muscovy ducklings, leading to a huge economic loss. However, little is known about the localization of B-cell epitopes on GPV structural protein. To address the issue, the structural protein of GPV was dissected into sets of partially overlapping fragments and expressed in Escherichia coli. Then Western blot reactivity of these glutathione S-transferase (GST) fusion short peptides to viral infected sera was surveyed. The results showed linear immunodominant epitopes, which were found in seven fragments covering amino acid residues 35-71, 123-198, 423-444, 474-491, 531-566, 616-669, 678-732. Our findings may provide the basis for the development of immunity-based prophylactic, therapeutic, and diagnostic clinical techniques for Derzsy's disease.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Proteínas Estruturais Virais
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Parvovirus
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Infecções por Parvoviridae
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Epitopos de Linfócito B
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Gansos
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article